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LentiVirusStocks

nr name titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 shisa9_FLAG_ires_EGFP   18.10.2012 Anna 5 ultracentrifuge UD1  
2 Caspr2_shRNA_70   2012-10-18 Ning 5 and 10 ultracentrifuge UD1  
3 Caspr2_shRNA_72   2012-10-18 Ning 5 and 10 ultracentrifuge UD1  
4 Caspr2_shRNA_73   2012-10-18 Ning 5 and 10 ultracentrifuge UD1  
5 GNB5_shRNA_1   2012-10-18 Ramesh 5 and10 ultracentrifuge UD1  
6 GNB5_shRNA_3   2012-10-18 Ramesh 5 and 10 ultracentrifuge UD1  
7 Nedd4-1 WT IRES EGFP   2013-09-26 Anna 6 ultracentrifuge UD1 Nedd4-1 WT has 6 point mutations, plasmid obtained from Olivier Staub lab
8 Nedd4-1 CS catalytically inactive IRES EGFP   2013-09-26 Anna 6 ultracentrifuge UD1 Nedd4-1 CS has 6 point mutations, plasmid obtained from Olivier Staub lab
9 Ex_pLV_Syn(pr.)ires2EGFPLL3_shisa6 (#Yv01)   2014-10-30 Yvonne   ultracentrifuge UD1 Good transfection
10 Ex_pLV_Syn(pr.)ires2EGFPLL3_shisa7 (#Yv02)   2014-10-30 Yvonne   ultracentrifuge UD1 Good transfection
11 pLV_Syn(pr.)_EGFP (#Yv24)   2014-12-04 Yvonne 5 ul ultracentrifuge UD1 Good transfection
12 Ex_pLV_Syn(pr.)ires2EGFPLL3_nrg3 (#Yv22)   2014-12-04 Yvonne 10 ul ultracentrifuge UD1 Good transfection, green cells difficult to see, but they were there
13 Cg6_T_V5pLenti (#Yv25)   2014-12-18 Yvonne 10 ul ultracentrifuge UD1 no green cells (=OK)
14 pEx_pLV_ires_EGFP(RZ extra C)_mCpt1 (#Yv23)   2014-12-18 Yvonne 10 ul ultracentrifuge UD1 no green cells with the transfection (?)
15 pEx_pTRIP_mPGK_Shisa 6_ires_EGFP (#Yv30)   2015-01-12 Yvonne 10 ul ultracentrifuge UD1 ++ green cells with transfection
16 pEx_pTRIP_mPGK_Shisa7_ires_EGFP (#Yv31)   2015-01-12 Yvonne 10 ul ultracentrifuge UD1 ++ green cells with transfection
17 pEx_pLV_ires_EGFP_mCpt1 (#Yv23)   2015-01-12 Yvonne 10 ul ultracentrifuge UD1 no green cells with the transfection (?)
18 Cg6_T_V5pLenti (#Yv32)   2015-01-12 Yvonne 10 ul ultracentrifuge UD1 no green cells (=OK)
19             UD1  
20             UD1  
21 LV_sh1 ++ 20/10/08 Yvonne     UD2  
22 LV_sh13 ++ 20/10/08 Yvonne     UD2  
23 pEx_Syn(pr.)_nrg3_ires_EGFP (clone #8 Elena)   2015-03-31 Yvonne 10 ul ultracentrifuge UD2 green cells with transfection
24             UD2  
25 LV_HcLuc           UD2  
26 LV_EBPRE_Luc     Harold 10   UD2  
27 pCMV_mPERK1(WT)-EGFP-N1_lenti #19   2015-03-31 Fabian 10 ul ultracentrifuge UD2  
28 pCMV_mPERK1(Y018A )-EGFP-N1_lenti #25   2015-03-31 Fabian 10 ul ultracentrifuge UD2  
29 pLV_CG6_T_V5   2015-06-04 Yvonne   ultracentrifuge UD2  
30 pEx_LV_6xMyc_cpt1c_ires_EGFP 2.2*10^8 2015-06-04 Yvonne   ultracentrifuge UD2  
31 NG5_HA_ires_EGFP 8.0*10^8 2015-06-04 Yvonne   ultracentrifuge UD2  
32 pLV_ires_EGFP 1.2*10^6 2015-06-04 Yvonne   ultracentrifuge UD2  
33 pEx_pLV_CMV_CT84   2015-07-02 Yvonne 5+10 ultracentrifuge UD2 Step 1 of harvesting: rn1000xg 5 min; centrifugated 3200xg
34 pEx_pLV_Syn_CT84   2015-07-02 Yvonne 5+10 ultracentrifuge UD2 Step 1 of harvesting: rn1000xg 5 min; centrifugated 3200xg
35 pEx_pLV_CMV_CT100   2015-07-02 Yvonne 5+10 ultracentrifuge UD2 Step 1 of harvesting: rn1000xg 5 min; centrifugated 3200xg
36 pEx_pLV_Syn_CT100   2015-07-02 Yvonne 5+10 ultracentrifuge UD2 Step 1 of harvesting: rn1000xg 5 min; centrifugated 3200xg
37 pLV_ires_EGFP   2015-07-02 Yvonne 5+10 ultracentrifuge UD2 Step 1 of harvesting: rn1000xg 5 min; centrifugated 3200xg
38             UD2  
39             UD3  
40 sh1_CG6   160212 Yvonne 50 amicon filter UD3  
41 sh2_CG6   160212 Yvonne 50 amicon filter UD3  
42 sh3_CG6   160212 Yvonne 50 amicon filter UD3  
43 shC   160212 Yvonne 50 amicon filter UD3  
44 sh1_GPR156   160212 Yvonne 50 amicon filter UD3  
45 sh2_GPR156   160212 Yvonne 50 amicon filter UD3  
46 sh3_GPR156   160212 Yvonne 50 amicon filter UD3  
47 sh4_GPR156   160212 Yvonne 50 amicon filter UD3  
48 NG5_WT   050312 Yvonne 10 ultracentrifuge UD3  
49 NG5_HA   050312 Yvonne 10 ultracentrifuge UD3  
50 GPR156_OE   050312 Yvonne 10 ultracentrifuge UD3  
51 sh2_CG6   050312 Yvonne 20 ultracentrifuge UD3  
52 pLenti_sh6 (aip2)   050312 Yvonne 20 ultracentrifuge UD3  
53 pLenti_sh12 (aip4)   050312 Yvonne 20 ultracentrifuge UD3  
54 shisa6_FLAG_ires_EGFP 3*10^7 190612 Yvonne 5 and 10 (blue dot) ultracentrifuge UD3  
55 shisa7_FLAG_ires_EGFP 7*10^8 190612 Yvonne 5 and 10 (blue dot) ultracentrifuge UD3  
56 shisa 7_K/R_ires_EGFP 8*10^8 190612 Yvonne 5 and 10 (blue dot) ultracentrifuge UD4  
57 shisa 9_HA_ires_EGFP 4,4*10^8 190612 Yvonne 5 and 10 (blue dot) ultracentrifuge UD4  
58 shAIP2AB.3 (sh6 against Shisa 6) ~ 5*10^7 200812 Yvonne 10 ultracentrifuge UD4  
59 shAIP4AB.1 (sh12 against Shisa 7) ~ 5*10^7 200812 Yvonne 10 ultracentrifuge UD4  
60 shC (against plant arobidopsis) ~ 5*10^7 200812 Yvonne 10 ultracentrifuge UD4  
61 FAMshRNA1 (Nikhyl)   170912 Yvonne 10 ultracentrifuge UD4  
62 FAMshRNA2 (Nikhyl)   170912 Yvonne 10 ultracentrifuge UD4  
63 FAMshRNA3 (Nikhyl)   170912 Yvonne 10 ultracentrifuge UD4  
64 FAMshRNA4 (Nikhyl)   170912 Yvonne 10 ultracentrifuge UD4  
65 FAMshRNA5 (Nikhyl)   170912 Yvonne 10 ultracentrifuge UD4  
66 CG6_UT_pDEST_LENTI_IRES_GFP (Nikhyl)   170912 Nikhyl 10 ultracentrifuge UD4  
67 pEx_pLenti_Trim3_ires_EGFP 10.9*10^9 2014-09-12 Yvonne 10 ultracentrifuge UD4  
68 pEx_pLenti_Trim3_dRBCC_ires_EGFP 11.7*10^9 2014-09-12 Yvonne 10 ultracentrifuge UD4  
69 pEx_pLentiSyn(pr.)_nrg3_ires_EGFP no green HEK 2014-09-12 Yvonne 10 ultracentrifuge UD4  
70 pLenti_CMV_cre_EGFP 7.4*10^8 2014-09-12 Yvonne 10 ultracentrifuge UD4  
Topic revision: r22 - 2015-07-02 - 13:21:01 - YvonneGouwenberg
 
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