Difference: AAVVirusStocks (1 vs. 45)

Revision 452016-02-09 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 41 to 41
 
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       UE2box 4  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   UE2box 4  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   UE2box 4  
Changed:
<
<
37   AAV2           UE2box 5  
38   AAV2           UE2box 5  
39   AAV2           UE2box 5  
>
>
37 AAV-GFAP::HA-hM3Dq-IRES-mCitrine AAV2 1.0*10^12 09-01-16 Michel 25   UE2box 5 UNC vector core LOT AV4748
38 AAV-GFAP::HA-hM4Di-IRES-mCitrine AAV2 4.0*10^12 09-01-16 Michel 25   UE2box 5 UNC vector core LOT AV4749
39 AAV-GFAP::HA-hM3Ds-IRES-mCitrine AAV2 4.0*10^12 09-01-16 Michel 25   UE2box 5 UNC vector core LOT AV4750
 
40   AAV2           UE2box 5  
41   AAV2           UE2box 5  
42 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 4*10^10 4 dec '10 Yvonne 25 ul   UE2box 5  
Line: 119 to 119
 
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
113 AAV-hSyn-DIO-mCherry AAV5 5.2E+12 2015-08-10 Michel 25uL   UE4box 13 UNC vector core LOT AV4634C
114 pAAV-CAMKII-HA-KORD-IRES-mCitrine AAV9 3.5E+12 2015-12-04 Michel 25uL   UE4box 13 UNC vector core LOT AV5208B
Changed:
<
<
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9 5.3E+12 2015-01-26 Michel 25uL   UE4box13 UNC vector core LOT AV5209B
>
>
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9 5.3E+12 2015-01-26 Michel 25uL     UNC vector core LOT AV5209B
116   AAV1              
  \ No newline at end of file

Revision 442016-02-04 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 119 to 119
 
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
113 AAV-hSyn-DIO-mCherry AAV5 5.2E+12 2015-08-10 Michel 25uL   UE4box 13 UNC vector core LOT AV4634C
114 pAAV-CAMKII-HA-KORD-IRES-mCitrine AAV9 3.5E+12 2015-12-04 Michel 25uL   UE4box 13 UNC vector core LOT AV5208B
Changed:
<
<
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9     Michel        
>
>
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9 5.3E+12 2015-01-26 Michel 25uL   UE4box13 UNC vector core LOT AV5209B
  \ No newline at end of file

Revision 432015-12-04 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 118 to 118
 
111 AAV-hsyn-DIO-hM4D(Gi)-mCherry AAV5 6,0E+12 2015-03-19 Michel 25uL   UE4box 13 UNC vector core
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
113 AAV-hSyn-DIO-mCherry AAV5 5.2E+12 2015-08-10 Michel 25uL   UE4box 13 UNC vector core LOT AV4634C
Changed:
<
<
114 pAAV-CAMKII-HA-KORD-IRES-mCitrine AAV9     Michel        
>
>
114 pAAV-CAMKII-HA-KORD-IRES-mCitrine AAV9 3.5E+12 2015-12-04 Michel 25uL   UE4box 13 UNC vector core LOT AV5208B
 
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9     Michel        

Revision 422015-11-27 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     UE1box 1  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     UE1box 1  
Line: 118 to 118
 
111 AAV-hsyn-DIO-hM4D(Gi)-mCherry AAV5 6,0E+12 2015-03-19 Michel 25uL   UE4box 13 UNC vector core
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
113 AAV-hSyn-DIO-mCherry AAV5 5.2E+12 2015-08-10 Michel 25uL   UE4box 13 UNC vector core LOT AV4634C
Added:
>
>
114 pAAV-CAMKII-HA-KORD-IRES-mCitrine AAV9     Michel        
115 pAAV-hSyn-DIO-HA-KORD-IRES-mCitrine AAV9     Michel        
  \ No newline at end of file

Revision 412015-09-21 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 14 to 14
 
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     UE1box 1  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     UE1box 1  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     UE1box 1  
Changed:
<
<
10 TRE::Cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 TRE::Cre-EGFP AAV2   2015-01-27 Mariana 25   UE1box 2  
12 TRE::Cre-ERT2 AAV2   2015-01-27 Mariana 25   UE1box 2  
>
>
10 AAV-TRE::Cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 AAV-TRE::Cre-EGFP AAV2   2015-01-27 Mariana 25   UE1box 2  
12 AAV-TRE::Cre-ERT2 AAV2   2015-01-27 Mariana 25   UE1box 2  
 
13 fSST-RFP AAV2   2015-07-03 Yvonne 25   UE1box 2  
Changed:
<
<
14 ptetbi-iCre-tdTOM AAV2   2015-08-27 Mariana 25   UE1box 2  
>
>
14 AAV-Ptetbi-iCre-tdTOM AAV2   2015-08-27 Mariana 25   UE1box 2  
 
15   AAV2           UE1box 2  
16   AAV5       30 ul   UE1box 2  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   UE1box 2  
Line: 66 to 66
 
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 op
60 pAAV_DIO_ChR2-EYFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   UE2box 7 op
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   UE2box 7  
Changed:
<
<
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   UE2box 7  
>
>
62 AAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   UE2box 7  
 
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   UE2box 7 op
64 AAV_CamKII_hChR2(H134R )-EYFP AAV2 3.26E+13 13/10/11 Yvonne 25 ul   UE3box 8 op
65 AAV_CamKII_icreEGFP2aCREB AAV2 5.06E+12 13/10/11 Yvonne 25 ul   UE3box 8  
Line: 105 to 105
 
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   UE4box 11.1 2 plates (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   UE4box 11.1 UNC Vector Core
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit.
Changed:
<
<
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit. MVDO: no expression with 0.5ul in mPFC
102 AAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d); MVDO: Good expression, e-phys data variable
104 AAV_CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 MVDO: good expression with 0.5 and 1.0 ul in mPFC
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence. MVDO: Reasonable expression with 1.0ul
>
>
101 AAV-ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit. MVDO: no expression with 0.5ul in mPFC
102 AAV-TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV-CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d); MVDO: Good expression, e-phys data variable
104 AAV-CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 MVDO: good expression with 0.5 and 1.0 ul in mPFC
105 AAV-TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence. MVDO: Reasonable expression with 1.0ul
 
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core. MVDO: Good expression
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core. MVDO: good expression
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection

Revision 402015-08-27 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 17 to 17
 
10 TRE::Cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 TRE::Cre-EGFP AAV2   2015-01-27 Mariana 25   UE1box 2  
12 TRE::Cre-ERT2 AAV2   2015-01-27 Mariana 25   UE1box 2  
Changed:
<
<
13   AAV5           UE1box 2  
14   AAV2           UE1box 2  
>
>
13 fSST-RFP AAV2   2015-07-03 Yvonne 25   UE1box 2  
14 ptetbi-iCre-tdTOM AAV2   2015-08-27 Mariana 25   UE1box 2  
 
15   AAV2           UE1box 2  
16   AAV5       30 ul   UE1box 2  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   UE1box 2  

Revision 392015-08-10 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 117 to 117
 
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core. MVDO: Good expression. Fos induction after CNO
111 AAV-hsyn-DIO-hM4D(Gi)-mCherry AAV5 6,0E+12 2015-03-19 Michel 25uL   UE4box 13 UNC vector core
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
Added:
>
>
113 AAV-hSyn-DIO-mCherry AAV5 5.2E+12 2015-08-10 Michel 25uL   UE4box 13 UNC vector core LOT AV4634C
  \ No newline at end of file

Revision 382015-03-25 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 116 to 116
 
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core. MVDO: Good expression. Fos induction after CNO
111 AAV-hsyn-DIO-hM4D(Gi)-mCherry AAV5 6,0E+12 2015-03-19 Michel 25uL   UE4box 13 UNC vector core
Added:
>
>
112 AAV-CAMKII::EYFP AAV5 4.0E+12   Michel     UE4box 13 UNC vector core LOT AV4808
 

Revision 372015-03-24 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 14 to 14
 
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     UE1box 1  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     UE1box 1  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     UE1box 1  
Changed:
<
<
10 TRE_cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 cre_gfp AAV2   2015-01-27 Mariana 25   UE1box 2  
12 cre_ERT AAV2   2015-01-27 Mariana 25   UE1box 2  
>
>
10 TRE::Cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 TRE::Cre-EGFP AAV2   2015-01-27 Mariana 25   UE1box 2  
12 TRE::Cre-ERT2 AAV2   2015-01-27 Mariana 25   UE1box 2  
 
13   AAV5           UE1box 2  
14   AAV2           UE1box 2  
15   AAV2           UE1box 2  

Revision 362015-03-19 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 115 to 115
 
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core. MVDO: Good expression. Fos induction after CNO
Added:
>
>
111 AAV-hsyn-DIO-hM4D(Gi)-mCherry AAV5 6,0E+12 2015-03-19 Michel 25uL   UE4box 13 UNC vector core
  \ No newline at end of file

Revision 352015-03-10 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 115 to 115
 
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core. MVDO: Good expression. Fos induction after CNO
Deleted:
<
<
111 AAV-TRE-Cre AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, Cre-recombination in HEK293 cells
112 AAV-TRE-Cre-GFP AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, green cells, Cre-recombination in HEK293 cells
113 AAV-TRE-CreERT AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, Cre-recombination in HEK293 cells
  \ No newline at end of file

Revision 342015-02-27 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 109 to 109
 
102 AAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d); MVDO: Good expression, e-phys data variable
104 AAV_CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 MVDO: good expression with 0.5 and 1.0 ul in mPFC
Changed:
<
<
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence
>
>
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence. MVDO: Reasonable expression with 1.0ul
 
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core. MVDO: Good expression
Changed:
<
<
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
>
>
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core. MVDO: good expression
 
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
Changed:
<
<
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core
111 AAV-TRE-CRE AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection
112 AAV-TRE-Cre-GFP AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection, green cells
113 AAV-TRE-Cre-ERT AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection
>
>
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core. MVDO: Good expression. Fos induction after CNO
111 AAV-TRE-Cre AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, Cre-recombination in HEK293 cells
112 AAV-TRE-Cre-GFP AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, green cells, Cre-recombination in HEK293 cells
113 AAV-TRE-CreERT AAV2   2015-01-30 Mariana 25uL     MM: 2 plates, good transfection, Cre-recombination in HEK293 cells
 

Revision 332015-02-25 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 115 to 115
 
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core
Added:
>
>
111 AAV-TRE-CRE AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection
112 AAV-TRE-Cre-GFP AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection, green cells
113 AAV-TRE-Cre-ERT AAV2   2015-01-30 Mariana 25uL     2 plates, good transfection
  \ No newline at end of file

Revision 322015-02-03 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 14 to 14
 
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     UE1box 1  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     UE1box 1  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     UE1box 1  
Changed:
<
<
10   AAV8           UE1box 2  
11   AAV2           UE1box 2  
12   AAV8           UE1box 2  
>
>
10 TRE_cre AAV2   2015-01-27 Mariana 25   UE1box 2  
11 cre_gfp AAV2   2015-01-27 Mariana 25   UE1box 2  
12 cre_ERT AAV2   2015-01-27 Mariana 25   UE1box 2  
 
13   AAV5           UE1box 2  
14   AAV2           UE1box 2  
15   AAV2           UE1box 2  

Revision 312015-01-06 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 59 to 59
 
52 pDEST_pTRCGW_AIP2_IRES_EGFP AAV2 1,42E+11 14-Apr-11 Yvonne 25 ul   UE2box 6  
53 pDEST_pTRCGW_AIP4_IRES_EGFP AAV2 6,21E+11 14-Apr-11 Yvonne 25 ul   UE2box 6  
54 pDEST_pTRCGW_Myc_BCAN AAV2 1,43E+12 14-Apr-11 Yvonne 25 ul   UE2box 6 aliquot left
Changed:
<
<
55 AAV_DIO_Nphr_YFP AAV2 3,79E+12 14-Apr-11 Yvonne 25 ul   UE2box 7  
56 AAV_DIO_YFP AAV2 4,90E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 aliquot left
>
>
55 AAV_DIO_eNpHR3.0-EYFP AAV2 3,79E+12 14-Apr-11 Yvonne 25 ul   UE2box 7  
56 AAV_DIO_EYFP AAV2 4,90E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 aliquot left
 
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2,78E+12 14-Apr-11 Yvonne 25 ul   UE2box 7  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6,69E+12 14-Apr-11 Yvonne 25 ul   UE2box 7 op
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 op
Changed:
<
<
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   UE2box 7 op
>
>
60 pAAV_DIO_ChR2-EYFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   UE2box 7 op
 
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   UE2box 7  
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   UE2box 7  
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   UE2box 7 op
Line: 96 to 96
 
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4841)
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul   UE3box 11  
Changed:
<
<
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   UE3box 11  
>
>
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   UE3box 11 MVDO: Not good
 
93 AAV_CMV2_TRE_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   UE3box 11  
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   UE3box 11 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   UE3box 11 4 plates, combined after filter step
Line: 104 to 104
 
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   UE4box 11.1 2 platen (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   UE4box 11.1 2 plates (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   UE4box 11.1 UNC Vector Core
Changed:
<
<
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
>
>
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit.
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit. MVDO: no expression with 0.5ul in mPFC
 
102 AAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
Changed:
<
<
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d)
104 AAV_CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12  
>
>
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d); MVDO: Good expression, e-phys data variable
104 AAV_CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 MVDO: good expression with 0.5 and 1.0 ul in mPFC
 
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence
Changed:
<
<
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
>
>
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core. MVDO: Good expression
 
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection

Revision 302014-11-27 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 108 to 108
 
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
102 AAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d)
Changed:
<
<
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection
>
>
104 AAV_CMV_Myc_BCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12  
 
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core

Revision 292014-10-22 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 106 to 106
 
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   UE4box 11.1 UNC Vector Core
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
Changed:
<
<
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
>
>
102 AAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
 
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d)
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
Changed:
<
<
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
>
>
107 AAV-EF1a-DIO-EYFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
 
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
Changed:
<
<
110 hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core
>
>
110 AAV-hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core
 

Revision 282014-10-14 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 17 to 17
 
10   AAV8           UE1box 2  
11   AAV2           UE1box 2  
12   AAV8           UE1box 2  
Changed:
<
<
13   AAV5   5/4/2009       UE1box 2  
>
>
13   AAV5           UE1box 2  
 
14   AAV2           UE1box 2  
15   AAV2           UE1box 2  
16   AAV5       30 ul   UE1box 2  
Line: 89 to 89
 
82 AAV_DIO_YFP AAV2 1,03E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 3 tubes, little bit to much concentrated,
83 AAV_DIO_ChR2_YFP AAV2 1,39E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 2 tubes, little bit to much concentrated
84 AAV_CamKII_eNpHR3.0_EYFP AAV2 1,95E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 1 tube, little bit to much concentrated
Changed:
<
<
85 Leanne AAV2           UE3box 10 Leanne
86 Leanne AAV1           UE3box 10 Leanne
87 Leanne AAV1           UE3box 10 Leanne
>
>
85   AAV2           UE3box 10  
86   AAV1           UE3box 10  
87   AAV1           UE3box 10  
 
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17 Michel     UE3box 10 UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4841)

Revision 272014-10-10 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     UE1box 1  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     UE1box 1  
Line: 14 to 14
 
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     UE1box 1  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     UE1box 1  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     UE1box 1  
Changed:
<
<
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     UE1box 2  
>
>
10   AAV8           UE1box 2  
 
11   AAV2           UE1box 2  
Changed:
<
<
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   UE1box 2  
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     UE1box 2  
>
>
12   AAV8           UE1box 2  
13   AAV5   5/4/2009       UE1box 2  
 
14   AAV2           UE1box 2  
15   AAV2           UE1box 2  
Changed:
<
<
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   UE1box 2  
>
>
16   AAV5       30 ul   UE1box 2  
 
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   UE1box 2  
Changed:
<
<
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   UE1box 2  
19 CMV_FRZB_IRES_GFP AAV1   7/23/2009 Maarten 25 ul   UE1box 3  
20 CMV_IRES_EGFP (Maarten) AAV2 6,1*10^11 7/23/2009 Yvonne 25 ul   UE1box 3  
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   UE1box 3  
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   UE1box 3  
23 pTRCGW_shAIP4_CMV_GFP (sh12) AAV8 2,2*10^11 9/22/2009 Yvonne 10/25 ul   UE1box 3  
>
>
18   AAV5       50 ul   UE1box 2  
19 CMV_FRZB_IRES_GFP AAV1   7/23/2009 Maarten 25 ul   UE1box 3.1  
20   AAV2       25 ul   UE1box 3.1  
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   UE1box 3.1  
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   UE1box 3.1  
23 pTRCGW_shAIP4_CMV_GFP (sh12) AAV8 2,2*10^11 9/22/2009 Yvonne 10/25 ul   UE1box 3.1  
 
24 CMV_IRES_EGFP (Maarten) AAV5 1,5*10^13 3/5/2010 Connie 25 ul   UE1box 3  
25 CMV_SREBP2_IRES_EGFP AAV5 3,0*10^13 3/5/2010 Connie 25 ul   UE1box 3  
26 CMV_Aip2_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   UE1box 3  
27 CMV_Aip4_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   UE1box 3  
Changed:
<
<
28 CMV_IRES_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
29 MBP_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
30 CMV_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
31 pAAV-6P-FLAG-Aip1 (volker) AAV2 2*10^10 7/22/2010 Yvonne 10 ul   UE1box 4  
32 pAAV-6P-HA-Aip1 (volker) AAV2 2,7*10^13 7/22/2010 Yvonne 10 ul   UE1box 4  
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10       UE1box 4  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       UE1box 4  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   UE1box 4  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   UE1box 4  
37 AAV-Dio-ChR2-mCherry AAV2 1,8*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag
38 AAV-Dio-ChR2-mCherry AAV2 1,3*10^10 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
39 AAV-Dio-NpHR-EYFP AAV2 1,5*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag
40 AAV-Dio-NpHR-EYFP AAV2 5,5*10^9 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
41 CMV_IRES_EGFP AAV2 4,1*10^9 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
42 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 4*10^10 4 dec '10 Yvonne 25 ul   DD1  
43 CMV_IRES_EGFP AAV2 1,31 * 10^12 4 dec '10 Yvonne 25 ul   DD1 Bijna op
44 CMV_IRES_EGFP AAV2 8*10^11 4 dec '10 Yvonne 25 ul   DD1  
45 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 2,24*10^10 4 dec '10 Yvonne 25 ul   DD1  
46 AAV-Dio-ChR2-mCherry AAV2 2,22 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
47 AAV-Dio-NpHR-EYFP AAV2 2,01 * 10^12 4 dec '10 Yvonne 25 ul   DD1 is op
48 pDEST_pTRCGW_Nrg3 AAV2 3,15E+12 14-Apr-11 Yvonne 25 ul   DD1  
49 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 6,88E+11 14-Apr-11 Yvonne 25 ul   DD1  
50 pDEST_pTRCGW_Myc_Nrg3 AAV2 1,54E+12 14-Apr-11 Yvonne 25 ul   DD1  
51 pDEST_pTRCGW_Myc_Nrg3_IRES_EGFP AAV2 3,85E+11 14-Apr-11 Yvonne 25 ul   DD1  
52 pDEST_pTRCGW_AIP2_IRES_EGFP AAV2 1,42E+11 14-Apr-11 Yvonne 25 ul   DD1  
53 pDEST_pTRCGW_AIP4_IRES_EGFP AAV2 6,21E+11 14-Apr-11 Yvonne 25 ul   DD1  
54 pDEST_pTRCGW_Myc_BCAN AAV2 1,43E+12 14-Apr-11 Yvonne 25 ul   DD1  
55 AAV_DIO_Nphr_YFP AAV2 3,79E+12 14-Apr-11 Yvonne 25 ul   DD1  
56 AAV_DIO_YFP AAV2 4,90E+13 14-Apr-11 Yvonne 25 ul   DD1  
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2,78E+12 14-Apr-11 Yvonne 25 ul   DD1  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6,69E+12 14-Apr-11 Yvonne 25 ul   DD1  
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   DD1  
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   DD1 is op
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   DD1  
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   DD1  
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   DD1 is op
64 AAV_CamKII_hChR2(H134R )-EYFP AAV2 3.26E+13 13/10/11 Yvonne 25 ul   DD1  
65 AAV_CamKII_icreEGFP2aCREB AAV2 5.06E+12 13/10/11 Yvonne 25 ul   DD1  
66 AAV_CamKII_CREB_GFP_IRES_cre AAV2 4.94E+13 13/10/11 Yvonne 25 ul   DD2  
67 AAV_DIO_ChR2_YFP AAV2 4.83E+11 120312 Yvonne 25 ul   DD2  
68 AAV_CamKII_eNpHR3.0_EYFP AAV2 6.46E+11 120312 Yvonne 25 ul   DD2  
69 AAV_CamKII_CREB_GFP_ires_cre AAV2 1.98E+11 120312 Yvonne 25 ul   DD2  
70 AAV_CamKII_GFP_ires_cre AAV2 4.90E+09 120312 Yvonne 25 ul   DD2 weg, titer te laag
71 AAV_CamKII_GFP_ires_cre AAV2 1.44E+13 250612 Yvonne 25 ul   DD2  
72 AAV_CamKII_CREB_GFP_ires_cre AAV2 6.50E+12 250612 Yvonne 25 ul   DD2  
73 pDEST_pTRCGW_Myc_BCAN AAV2 3.20E+10 250612 Yvonne 25 ul   DD2  
74 pDEST_pTRCGW_Myc_BCAN AAV2 5.40E+10 250612 Yvonne 25 ul   DD2  
75 AAV_CamKII_GFP_ires_cre AAV2 2.75E+11 250612 Yvonne 25 ul   DD2  
76 AAV_CamKII_GFP_ires_cre AAV2 5.60E+10 250612 Yvonne 25 ul   DD2  
77 AAV_shisa6_ires_EGFP AAV2 1.11E+10 200712 Yvonne 25 ul   DD2  
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   DD2  
79 AAV_shisa6_ires_EGFP AAV2 1,67E+10 2012-08-20 Yvonne 25 ul   DD2  
80 AAV_shisa7_ires_EGFP AAV2 1,18E+11 2012-08-20 Yvonne 25 ul   DD2  
81 pTRCGW_nrg3_ires2_EGFP AAV2 2,81E+12 2012-10-23 Yvonne 25 ul   DD3 only 2 tubes, little bit to much concentrated
82 AAV_DIO_YFP AAV2 1,03E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 3 tubes, little bit to much concentrated,
83 AAV_DIO_ChR2_YFP AAV2 1,39E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 2 tubes, little bit to much concentrated
84 AAV_CamKII_eNpHR3.0_EYFP AAV2 1,95E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 1 tube, little bit to much concentrated
85 Leanne AAV2             Leanne
86 Leanne AAV1             Leanne
87 Leanne AAV1             Leanne
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17 Michel       UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4841)
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
93 AAV_CMV2_TRE_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3  
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 platen (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 plates (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   DD3 UNC Vector Core
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   DD4 UNC vector Core (Lot AV4617d)
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection, lots of red fluorescence
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
110 hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   DD4 UNC vector core
>
>
28 CMV_IRES_GFP AAV1   5/18/2010 Connie 25 ul   UE2box 4  
29 MBP_GFP AAV1   5/18/2010 Connie 25 ul   UE2box 4  
30 CMV_GFP AAV1   5/18/2010 Connie 25 ul   UE2box 4  
31 pAAV-6P-FLAG-Aip1 (volker) AAV2 2*10^10 7/22/2010 Yvonne 10 ul   UE2box 4  
32 pAAV-6P-HA-Aip1 (volker) AAV2 2,7*10^13 7/22/2010 Yvonne 10 ul   UE2box 4  
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10       UE2box 4  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       UE2box 4  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   UE2box 4  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   UE2box 4  
37   AAV2           UE2box 5  
38   AAV2           UE2box 5  
39   AAV2           UE2box 5  
40   AAV2           UE2box 5  
41   AAV2           UE2box 5  
42 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 4*10^10 4 dec '10 Yvonne 25 ul   UE2box 5  
43 CMV_IRES_EGFP AAV2 1,31 * 10^12 4 dec '10 Yvonne 25 ul   UE2box 5 op
44 CMV_IRES_EGFP AAV2 8*10^11 4 dec '10 Yvonne 25 ul   UE2box 5  
45 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 2,24*10^10 4 dec '10 Yvonne 25 ul   UE2box 5  
46 AAV-Dio-ChR2-mCherry AAV2 2,22 * 10^12 4 dec '10 Yvonne 25 ul   UE2box 6  
47 AAV-Dio-NpHR-EYFP AAV2 2,01 * 10^12 4 dec '10 Yvonne 25 ul   UE2box 6 op
48 pDEST_pTRCGW_Nrg3 AAV2 3,15E+12 14-Apr-11 Yvonne 25 ul   UE2box 6  
49 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 6,88E+11 14-Apr-11 Yvonne 25 ul   UE2box 6 alliqout left
50 pDEST_pTRCGW_Myc_Nrg3 AAV2 1,54E+12 14-Apr-11 Yvonne 25 ul   UE2box 6  
51 pDEST_pTRCGW_Myc_Nrg3_IRES_EGFP AAV2 3,85E+11 14-Apr-11 Yvonne 25 ul   UE2box 6  
52 pDEST_pTRCGW_AIP2_IRES_EGFP AAV2 1,42E+11 14-Apr-11 Yvonne 25 ul   UE2box 6  
53 pDEST_pTRCGW_AIP4_IRES_EGFP AAV2 6,21E+11 14-Apr-11 Yvonne 25 ul   UE2box 6  
54 pDEST_pTRCGW_Myc_BCAN AAV2 1,43E+12 14-Apr-11 Yvonne 25 ul   UE2box 6 aliquot left
55 AAV_DIO_Nphr_YFP AAV2 3,79E+12 14-Apr-11 Yvonne 25 ul   UE2box 7  
56 AAV_DIO_YFP AAV2 4,90E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 aliquot left
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2,78E+12 14-Apr-11 Yvonne 25 ul   UE2box 7  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6,69E+12 14-Apr-11 Yvonne 25 ul   UE2box 7 op
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   UE2box 7 op
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   UE2box 7 op
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   UE2box 7  
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   UE2box 7  
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   UE2box 7 op
64 AAV_CamKII_hChR2(H134R )-EYFP AAV2 3.26E+13 13/10/11 Yvonne 25 ul   UE3box 8 op
65 AAV_CamKII_icreEGFP2aCREB AAV2 5.06E+12 13/10/11 Yvonne 25 ul   UE3box 8  
66 AAV_CamKII_CREB_GFP_IRES_cre AAV2 4.94E+13 13/10/11 Yvonne 25 ul   UE3box 8 aliquot left
67 AAV_DIO_ChR2_YFP AAV2 4.83E+11 120312 Yvonne 25 ul   UE3box 8  
68 AAV_CamKII_eNpHR3.0_EYFP AAV2 6.46E+11 120312 Yvonne 25 ul   UE3box 8  
69 AAV_CamKII_CREB_GFP_ires_cre AAV2 1.98E+11 120312 Yvonne 25 ul   UE3box 8  
70   AAV2           UE3box 8  
71 AAV_CamKII_GFP_ires_cre AAV2 1.44E+13 250612 Yvonne 25 ul   UE3box 8  
72 AAV_CamKII_CREB_GFP_ires_cre AAV2 6.50E+12 250612 Yvonne 25 ul   UE3box 8  
73 pDEST_pTRCGW_Myc_BCAN AAV2 3.20E+10 250612 Yvonne 25 ul   UE3box 9  
74 pDEST_pTRCGW_Myc_BCAN AAV2 5.40E+10 250612 Yvonne 25 ul   UE3box 9  
75 AAV_CamKII_GFP_ires_cre AAV2 2.75E+11 250612 Yvonne 25 ul   UE3box 9  
76 AAV_CamKII_GFP_ires_cre AAV2 5.60E+10 250612 Yvonne 25 ul   UE3box 9  
77 AAV_shisa6_ires_EGFP AAV2 1.11E+10 200712 Yvonne 25 ul   UE3box 9  
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   UE3box 9  
79 AAV_shisa6_ires_EGFP AAV2 1,67E+10 2012-08-20 Yvonne 25 ul   UE3box 9  
80 AAV_shisa7_ires_EGFP AAV2 1,18E+11 2012-08-20 Yvonne 25 ul   UE3box 9  
81 pTRCGW_nrg3_ires2_EGFP AAV2 2,81E+12 2012-10-23 Yvonne 25 ul   UE3box 9 only 2 tubes, little bit to much concentrated
82 AAV_DIO_YFP AAV2 1,03E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 3 tubes, little bit to much concentrated,
83 AAV_DIO_ChR2_YFP AAV2 1,39E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 2 tubes, little bit to much concentrated
84 AAV_CamKII_eNpHR3.0_EYFP AAV2 1,95E+14 2012-10-23 Yvonne 25 ul   UE3box 10 DILUTE BEFORE USE!!! only 1 tube, little bit to much concentrated
85 Leanne AAV2           UE3box 10 Leanne
86 Leanne AAV1           UE3box 10 Leanne
87 Leanne AAV1           UE3box 10 Leanne
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17 Michel     UE3box 10 UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul   UE3box 10 UNC Vector Core (Lot AV4841)
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul   UE3box 11  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   UE3box 11  
93 AAV_CMV2_TRE_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   UE3box 11  
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   UE3box 11 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   UE3box 11 4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   UE3box 11 4 plates, combined after filter step
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   UE4box 11.1 2 platen (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   UE4box 11.1 2 plates (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   UE4box 11.1 UNC Vector Core
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   UE4box 12 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   UE4box 12 UNC vector Core (Lot AV4617d)
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   UE4box 12 2 plates, good transfection, lots of red fluorescence
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   UE4box 12 UNC vector core
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 12 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   UE4box 13 2 plates, good transfection
110 hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   UE4box 13 UNC vector core
 

Revision 262014-10-09 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
Changed:
<
<
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  
3 CMV_mGluR2 AAV5 3*10^10 1/29/2009 Danielle     DD7  
4 CMV_IRES_GFP(Harold) AAV5 5*10^11 1/29/2009 Dan/Maarten     DD7  
5 CMV_FRZB_IRES_GFP AAV5 1*10^12 1/29/2009 Maarten     DD7  
6 CMV_FRZB_IRES_GFP AAV2 2*10^11 3/13/2008 Maarten     DD7  
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     DD7  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     DD7  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     DD7  
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     DD7  
11   AAV2           DD7  
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   DD7  
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     DD7  
14   AAV2           DD7  
15   AAV2           DD7  
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   DD7  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   DD7  
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   DD7  
19 CMV_FRZB_IRES_GFP AAV1   7/23/2009 Maarten 25 ul   DD7  
20 CMV_IRES_EGFP (Maarten) AAV2 6,1*10^11 7/23/2009 Yvonne 25 ul   DD7  
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   DD7  
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   DD7  
23 pTRCGW_shAIP4_CMV_GFP (sh12) AAV8 2,2*10^11 9/22/2009 Yvonne 10/25 ul   DD7  
24 CMV_IRES_EGFP (Maarten) AAV5 1,5*10^13 3/5/2010 Connie 25 ul   DD7  
25 CMV_SREBP2_IRES_EGFP AAV5 3,0*10^13 3/5/2010 Connie 25 ul   DD7  
26 CMV_Aip2_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   DD7  
27 CMV_Aip4_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   DD7  
28 CMV_IRES_GFP AAV1   5/18/2010 Connie 25 ul   DD7  
29 MBP_GFP AAV1   5/18/2010 Connie 25 ul   DD7  
30 CMV_GFP AAV1   5/18/2010 Connie 25 ul   DD1  
31 pAAV-6P-FLAG-Aip1 (volker) AAV2 2*10^10 7/22/2010 Yvonne 10 ul   DD1  
32 pAAV-6P-HA-Aip1 (volker) AAV2 2,7*10^13 7/22/2010 Yvonne 10 ul   DD1  
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10       DD1  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       DD1  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   DD1  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   DD1  
>
>
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     UE1box 1  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     UE1box 1  
3 CMV_mGluR2 AAV5 3*10^10 1/29/2009 Danielle     UE1box 1  
4 CMV_IRES_GFP(Harold) AAV5 5*10^11 1/29/2009 Dan/Maarten     UE1box 1  
5 CMV_FRZB_IRES_GFP AAV5 1*10^12 1/29/2009 Maarten     UE1box 1  
6 CMV_FRZB_IRES_GFP AAV2 2*10^11 3/13/2008 Maarten     UE1box 1  
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     UE1box 1  
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     UE1box 1  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     UE1box 1  
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     UE1box 2  
11   AAV2           UE1box 2  
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   UE1box 2  
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     UE1box 2  
14   AAV2           UE1box 2  
15   AAV2           UE1box 2  
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   UE1box 2  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   UE1box 2  
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   UE1box 2  
19 CMV_FRZB_IRES_GFP AAV1   7/23/2009 Maarten 25 ul   UE1box 3  
20 CMV_IRES_EGFP (Maarten) AAV2 6,1*10^11 7/23/2009 Yvonne 25 ul   UE1box 3  
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   UE1box 3  
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   UE1box 3  
23 pTRCGW_shAIP4_CMV_GFP (sh12) AAV8 2,2*10^11 9/22/2009 Yvonne 10/25 ul   UE1box 3  
24 CMV_IRES_EGFP (Maarten) AAV5 1,5*10^13 3/5/2010 Connie 25 ul   UE1box 3  
25 CMV_SREBP2_IRES_EGFP AAV5 3,0*10^13 3/5/2010 Connie 25 ul   UE1box 3  
26 CMV_Aip2_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   UE1box 3  
27 CMV_Aip4_IRES_EGFP AAV2 ~ 10^9 4/20/2010 Yvonne 10 ul   UE1box 3  
28 CMV_IRES_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
29 MBP_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
30 CMV_GFP AAV1   5/18/2010 Connie 25 ul   UE1box 4  
31 pAAV-6P-FLAG-Aip1 (volker) AAV2 2*10^10 7/22/2010 Yvonne 10 ul   UE1box 4  
32 pAAV-6P-HA-Aip1 (volker) AAV2 2,7*10^13 7/22/2010 Yvonne 10 ul   UE1box 4  
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10       UE1box 4  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       UE1box 4  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   UE1box 4  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   UE1box 4  
 
37 AAV-Dio-ChR2-mCherry AAV2 1,8*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag
38 AAV-Dio-ChR2-mCherry AAV2 1,3*10^10 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
39 AAV-Dio-NpHR-EYFP AAV2 1,5*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag

Revision 252014-10-08 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 114 to 114
 
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
Added:
>
>
110 hsyn-DIO-hM3D(Gq)-mCherry AAV5 6,0E+12 2014-10-08 Michel 25 uL   DD4 UNC vector core
 

Revision 242014-09-15 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 112 to 112
 
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection, lots of red fluorescence
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
Added:
>
>
108 pDEST_pTRCGW_Trim3_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
109 pDEST_pTRCGW_Trim3_dRBCC_ires_EGFP AAV2   2014-09-15 Yvonne 25 ul   DD4 2 plates, good transfection
 

Revision 232014-09-11 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 110 to 110
 
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   DD4 UNC vector Core (Lot AV4617d)
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection, lots of red fluorescence
Added:
>
>
106 AAV-EF1a-mCherry-IRES-WGA-Cre AAV5 2,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
107 AAV-DIO-YFP AAV5 4,0E+12 2014-09-11 Michel 25 ul   DD4 UNC vector core
 

Revision 222014-07-10 - Main.MarianaMatos

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 108 to 108
 
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   DD4 UNC vector Core (Lot AV4617d)
Added:
>
>
104 AAV_CMV_Myc_BVCAN AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection
105 AAV_TRE_ChR2_mCherry AAV2   2014-07-01 Mariana 25 ul   DD4 2 plates, good transfection, lots of red fluorescence
  \ No newline at end of file

Revision 212014-07-10 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  

Revision 202014-05-12 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 107 to 107
 
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
Added:
>
>
103 AAV_CamKII_hM4D(Gi)_IRES_mCitrine AAV5   2014-05-12 Michel 25 ul   DD4 UNC vector Core (Lot AV4617d)
 \ No newline at end of file

Revision 192014-03-31 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 106 to 106
 
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   DD3 UNC Vector Core
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
Changed:
<
<
102 pAAV_TRE_Arch_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
>
>
102 pAAV_TRE_eArch3.0_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
 

Revision 182014-03-21 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  
Line: 101 to 101
 
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
Changed:
<
<
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 plates
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV1   2014-03-07 Yvonne 25 ul   DD3 2 plates
>
>
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 platen (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 plates (Robbie), heel veel drijvende cellen, medium gecentrifugeerd, wel veel groene cellen
 
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   DD3 UNC Vector Core
Added:
>
>
100 AAV_nrg3 (zonder extra kozak)_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
101 AAV_ires_EGFP 3 AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit
102 pAAV_TRE_Arch_EYFP AAV2   2014-03-21 Yvonne 25 ul   DD4 2 plates, transfectie zag er goed uit, groen signaal in het membraan
 \ No newline at end of file

Revision 172014-03-12 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 103 to 103
 
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 plates
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV1   2014-03-07 Yvonne 25 ul   DD3 2 plates
Added:
>
>
99 AAV_EF1a_DIO_ChR2(H134R )_EYFP AAV5   2014-03-12 Michel 25 ul   DD3 UNC Vector Core
 \ No newline at end of file

Revision 162014-03-07 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 101 to 101
 
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
Added:
>
>
97 AAV_ires_EGFP 2 AAV2   2014-03-07 Yvonne 25 ul   DD3 2 plates
98 AAV_nrg3 (zonder extra kozak)_ires_EGFP 2 AAV1   2014-03-07 Yvonne 25 ul   DD3 2 plates
 \ No newline at end of file

Revision 152014-02-14 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 99 to 99
 
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
93 AAV_CMV2_TRE_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3  
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined just before the last filter step
Changed:
<
<
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul     4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul     4 plates, combined after filter step
>
>
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined after filter step
 

Revision 142014-02-07 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 97 to 97
 
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4841)
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
Added:
>
>
93 AAV_CMV2_TRE_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3  
94 AAV_CMV2_TRE_ChR2_mCherry (2) AAV2   2014-02-07 Yvonne 25 ul   DD3 4 plates, combined just before the last filter step
95 AAV_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul     4 plates, combined after filter step
96 AAV_nrg3 (zonder extra kozak)_ires_EGFP AAV2   2014-02-07 Yvonne 25 ul     4 plates, combined after filter step
 \ No newline at end of file

Revision 132013-12-20 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 95 to 95
 
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17 Michel       UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4841)
Changed:
<
<
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul amicon filter DD3  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul amicon filter DD3  
>
>
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul   DD3  
 

Revision 122013-12-20 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  
Line: 92 to 92
 
85 Leanne AAV2             Leanne
86 Leanne AAV1             Leanne
87 Leanne AAV1             Leanne
Changed:
<
<
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17         UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17   25 ul     UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17   25 ul     UNC Vector Core (Lot AV4841)
>
>
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17 Michel       UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4316e)
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17 Michel 25 ul     UNC Vector Core (Lot AV4841)
91 AAV_CMV2_TRE_mCherry AAV2   2013-12-20 Yvonne 25 ul amicon filter DD3  
92 AAV_CMV2_TRE_ChR2_mCherry AAV2   2013-12-20 Yvonne 25 ul amicon filter DD3  
 \ No newline at end of file

Revision 112013-10-15 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 94 to 94
 
87 Leanne AAV1             Leanne
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17         UNC Vector Core (Lot AV4702)
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17   25 ul     UNC Vector Core (Lot AV4316e)
Added:
>
>
90 AAV_EF1a_DIO_eARCH3.0_EYFP AAV5 2,0E+12 2013-06-17   25 ul     UNC Vector Core (Lot AV4841)
 \ No newline at end of file

Revision 102013-07-23 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 93 to 93
 
86 Leanne AAV1             Leanne
87 Leanne AAV1             Leanne
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17         UNC Vector Core (Lot AV4702)
Added:
>
>
89 AAV_CaMKII_ChR2(H134R )_EYFP AAV5 4,0E+12 2013-06-17   25 ul     UNC Vector Core (Lot AV4316e)
 \ No newline at end of file

Revision 92013-06-18 - Main.MichelVanDenOever

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 89 to 89
 
82 AAV_DIO_YFP AAV2 1,03E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 3 tubes, little bit to much concentrated,
83 AAV_DIO_ChR2_YFP AAV2 1,39E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 2 tubes, little bit to much concentrated
84 AAV_CamKII_eNpHR3.0_EYFP AAV2 1,95E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 1 tube, little bit to much concentrated
Added:
>
>
85 Leanne AAV2             Leanne
86 Leanne AAV1             Leanne
87 Leanne AAV1             Leanne
88 AAV_CamKII_eARCH3.0_EYFP AAV5 4,0E+12 2013-06-17         UNC Vector Core (Lot AV4702)
 

Revision 82013-02-21 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 41 to 41
 
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       DD1  
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   DD1  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   DD1  
Changed:
<
<
37 AAV-Dio-ChR2-mCherry AAV2 1,8*10^10 5-11-10 Yvonne 25 ul   DD1  
38 AAV-Dio-ChR2-mCherry AAV2 1,3*10^10 12-11-10 Yvonne 25 ul   DD1  
39 AAV-Dio-NpHR-EYFP AAV2 1,5*10^10 5-11-10 Yvonne 25 ul   DD1  
40 AAV-Dio-NpHR-EYFP AAV2 5,5*10^9 12-11-10 Yvonne 25 ul   DD1  
41 CMV_IRES_EGFP AAV2 4,1*10^9 12-11-10 Yvonne 25 ul   DD1  
>
>
37 AAV-Dio-ChR2-mCherry AAV2 1,8*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag
38 AAV-Dio-ChR2-mCherry AAV2 1,3*10^10 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
39 AAV-Dio-NpHR-EYFP AAV2 1,5*10^10 5-11-10 Yvonne 25 ul   DD1 weg, titer te laag
40 AAV-Dio-NpHR-EYFP AAV2 5,5*10^9 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
41 CMV_IRES_EGFP AAV2 4,1*10^9 12-11-10 Yvonne 25 ul   DD1 weg, titer te laag
 
42 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 4*10^10 4 dec '10 Yvonne 25 ul   DD1  
Changed:
<
<
43 CMV_IRES_EGFP AAV2 1,31 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
>
>
43 CMV_IRES_EGFP AAV2 1,31 * 10^12 4 dec '10 Yvonne 25 ul   DD1 Bijna op
 
44 CMV_IRES_EGFP AAV2 8*10^11 4 dec '10 Yvonne 25 ul   DD1  
45 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 2,24*10^10 4 dec '10 Yvonne 25 ul   DD1  
46 AAV-Dio-ChR2-mCherry AAV2 2,22 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
Changed:
<
<
47 AAV-Dio-NpHR-EYFP AAV2 2,01 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
>
>
47 AAV-Dio-NpHR-EYFP AAV2 2,01 * 10^12 4 dec '10 Yvonne 25 ul   DD1 is op
 
48 pDEST_pTRCGW_Nrg3 AAV2 3,15E+12 14-Apr-11 Yvonne 25 ul   DD1  
49 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 6,88E+11 14-Apr-11 Yvonne 25 ul   DD1  
50 pDEST_pTRCGW_Myc_Nrg3 AAV2 1,54E+12 14-Apr-11 Yvonne 25 ul   DD1  
Line: 64 to 64
 
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2,78E+12 14-Apr-11 Yvonne 25 ul   DD1  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6,69E+12 14-Apr-11 Yvonne 25 ul   DD1  
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   DD1  
Changed:
<
<
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   DD1  
>
>
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   DD1 is op
 
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   DD1  
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   DD1  
Changed:
<
<
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   DD1  
>
>
63 AAV_CamKII_eNpHR3.0_EYFP AAV2 2.30E+14 13/10/11 Yvonne 25 ul   DD1 is op
 
64 AAV_CamKII_hChR2(H134R )-EYFP AAV2 3.26E+13 13/10/11 Yvonne 25 ul   DD1  
65 AAV_CamKII_icreEGFP2aCREB AAV2 5.06E+12 13/10/11 Yvonne 25 ul   DD1  
66 AAV_CamKII_CREB_GFP_IRES_cre AAV2 4.94E+13 13/10/11 Yvonne 25 ul   DD2  
67 AAV_DIO_ChR2_YFP AAV2 4.83E+11 120312 Yvonne 25 ul   DD2  
68 AAV_CamKII_eNpHR3.0_EYFP AAV2 6.46E+11 120312 Yvonne 25 ul   DD2  
69 AAV_CamKII_CREB_GFP_ires_cre AAV2 1.98E+11 120312 Yvonne 25 ul   DD2  
Changed:
<
<
70 AAV_CamKII_GFP_ires_cre AAV2 4.90E+09 120312 Yvonne 25 ul   DD2  
>
>
70 AAV_CamKII_GFP_ires_cre AAV2 4.90E+09 120312 Yvonne 25 ul   DD2 weg, titer te laag
 
71 AAV_CamKII_GFP_ires_cre AAV2 1.44E+13 250612 Yvonne 25 ul   DD2  
72 AAV_CamKII_CREB_GFP_ires_cre AAV2 6.50E+12 250612 Yvonne 25 ul   DD2  
73 pDEST_pTRCGW_Myc_BCAN AAV2 3.20E+10 250612 Yvonne 25 ul   DD2  

Revision 72012-11-02 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Line: 15 to 15
 
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     DD7  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     DD7  
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     DD7  
Changed:
<
<
11 WEG AAV1     Maarten     DD7  
>
>
11   AAV2           DD7  
 
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   DD7  
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     DD7  
Changed:
<
<
14 WEG AAV1           DD7  
15 WEG AAV1           DD7  
>
>
14   AAV2           DD7  
15   AAV2           DD7  
 
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   DD7  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   DD7  
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   DD7  
Line: 85 to 85
 
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   DD2  
79 AAV_shisa6_ires_EGFP AAV2 1,67E+10 2012-08-20 Yvonne 25 ul   DD2  
80 AAV_shisa7_ires_EGFP AAV2 1,18E+11 2012-08-20 Yvonne 25 ul   DD2  
Changed:
<
<
81 pTRCGW_nrg3_ires2_EGFP AAV2 2,81E+12 2012-10-23 Yvonne 25 ul      
>
>
81 pTRCGW_nrg3_ires2_EGFP AAV2 2,81E+12 2012-10-23 Yvonne 25 ul   DD3 only 2 tubes, little bit to much concentrated
82 AAV_DIO_YFP AAV2 1,03E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 3 tubes, little bit to much concentrated,
83 AAV_DIO_ChR2_YFP AAV2 1,39E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 2 tubes, little bit to much concentrated
84 AAV_CamKII_eNpHR3.0_EYFP AAV2 1,95E+14 2012-10-23 Yvonne 25 ul   DD3 DILUTE BEFORE USE!!! only 1 tube, little bit to much concentrated
 

Revision 62012-10-30 - Main.PimVanNierop

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  

Revision 52012-10-30 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  
Line: 83 to 83
 
76 AAV_CamKII_GFP_ires_cre AAV2 5.60E+10 250612 Yvonne 25 ul   DD2  
77 AAV_shisa6_ires_EGFP AAV2 1.11E+10 200712 Yvonne 25 ul   DD2  
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   DD2  
Changed:
<
<
79   AAV1              
>
>
79 AAV_shisa6_ires_EGFP AAV2 1,67E+10 2012-08-20 Yvonne 25 ul   DD2  
80 AAV_shisa7_ires_EGFP AAV2 1,18E+11 2012-08-20 Yvonne 25 ul   DD2  
81 pTRCGW_nrg3_ires2_EGFP AAV2 2,81E+12 2012-10-23 Yvonne 25 ul      
 

Revision 42012-10-29 - Main.YvonneGouwenberg

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

<-- /editTable -->
Deleted:
<
<
C CMV_GFP AAV2 2*10^11   Erich     DD7  
C1 CMV_GFP AAV2 3*10^11   Erich     DD7  
C2 CMV_GFP AAV8 3*10^11   Erich     DD7  
 
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7  
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7  
3 CMV_mGluR2 AAV5 3*10^10 1/29/2009 Danielle     DD7  
Line: 18 to 15
 
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     DD7  
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     DD7  
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     DD7  
Changed:
<
<
11 WEG       Maarten     DD7  
>
>
11 WEG AAV1     Maarten     DD7  
 
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   DD7  
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     DD7  
Changed:
<
<
14 WEG             DD7  
15 WEG             DD7  
>
>
14 WEG AAV1           DD7  
15 WEG AAV1           DD7  
 
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   DD7  
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   DD7  
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   DD7  
Changed:
<
<
19 CMV_FRZB_IRES_GFP     7/23/2009 Maarten 25 ul   DD7  
>
>
19 CMV_FRZB_IRES_GFP AAV1   7/23/2009 Maarten 25 ul   DD7  
 
20 CMV_IRES_EGFP (Maarten) AAV2 6,1*10^11 7/23/2009 Yvonne 25 ul   DD7  
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   DD7  
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   DD7  
Line: 40 to 37
 
30 CMV_GFP AAV1   5/18/2010 Connie 25 ul   DD1  
31 pAAV-6P-FLAG-Aip1 (volker) AAV2 2*10^10 7/22/2010 Yvonne 10 ul   DD1  
32 pAAV-6P-HA-Aip1 (volker) AAV2 2,7*10^13 7/22/2010 Yvonne 10 ul   DD1  
Changed:
<
<
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10 Robbie     DD1  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10 Robbie     DD1  
>
>
33 CMV_Flag_Aip2_IRES_EGFP AAV2 5,5*10^10 May-10       DD1  
34 CMV_Flag_Aip4_IRES_EGFP AAV2 1,5*10^11 May-10       DD1  
 
35 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,1*10^8 5-11-10 Yvonne 10 ul   DD1  
36 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 1,2*10^8 12-11-10 Yvonne 25 ul   DD1  
37 AAV-Dio-ChR2-mCherry AAV2 1,8*10^10 5-11-10 Yvonne 25 ul   DD1  
Line: 51 to 48
 
41 CMV_IRES_EGFP AAV2 4,1*10^9 12-11-10 Yvonne 25 ul   DD1  
42 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 4*10^10 4 dec '10 Yvonne 25 ul   DD1  
43 CMV_IRES_EGFP AAV2 1,31 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
Deleted:
<
<
43 (nieuwe QPCRmethode vanaf 28 juli) AAV2 7,02*10^13            
 
44 CMV_IRES_EGFP AAV2 8*10^11 4 dec '10 Yvonne 25 ul   DD1  
45 pTRCGW_pDEST_Myc_Brevican_IRES_EGFP AAV2 2,24*10^10 4 dec '10 Yvonne 25 ul   DD1  
46 AAV-Dio-ChR2-mCherry AAV2 2,22 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
47 AAV-Dio-NpHR-EYFP AAV2 2,01 * 10^12 4 dec '10 Yvonne 25 ul   DD1  
Changed:
<
<
48 pDEST_pTRCGW_Nrg3 AAV2 3148886086222.81 14-Apr-11 Yvonne 25 ul   DD1  
49 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 688413531696.155 14-Apr-11 Yvonne 25 ul   DD1  
50 pDEST_pTRCGW_Myc_Nrg3 AAV2 1535960459680.05 14-Apr-11 Yvonne 25 ul   DD1  
51 pDEST_pTRCGW_Myc_Nrg3_IRES_EGFP AAV2 385163462919.151 14-Apr-11 Yvonne 25 ul   DD1  
52 pDEST_pTRCGW_AIP2_IRES_EGFP AAV2 142335792184.881 14-Apr-11 Yvonne 25 ul   DD1  
53 pDEST_pTRCGW_AIP4_IRES_EGFP AAV2 620711254824.976 14-Apr-11 Yvonne 25 ul   DD1  
54 pDEST_pTRCGW_Myc_BCAN AAV2 1434400026941.57 14-Apr-11 Yvonne 25 ul   DD1  
55 AAV_DIO_Nphr_YFP AAV2 3791720189379.94 14-Apr-11 Yvonne 25 ul   DD1  
56 AAV_DIO_YFP AAV2 49033479323780.7 14-Apr-11 Yvonne 25 ul   DD1  
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2775605804092.32 14-Apr-11 Yvonne 25 ul   DD1  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6690965887286.52 14-Apr-11 Yvonne 25 ul   DD1  
59 pAAV-CamKII-EYFP AAV2 12629917363473.2 14-Apr-11 Yvonne 25 ul   DD1  
>
>
48 pDEST_pTRCGW_Nrg3 AAV2 3,15E+12 14-Apr-11 Yvonne 25 ul   DD1  
49 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 6,88E+11 14-Apr-11 Yvonne 25 ul   DD1  
50 pDEST_pTRCGW_Myc_Nrg3 AAV2 1,54E+12 14-Apr-11 Yvonne 25 ul   DD1  
51 pDEST_pTRCGW_Myc_Nrg3_IRES_EGFP AAV2 3,85E+11 14-Apr-11 Yvonne 25 ul   DD1  
52 pDEST_pTRCGW_AIP2_IRES_EGFP AAV2 1,42E+11 14-Apr-11 Yvonne 25 ul   DD1  
53 pDEST_pTRCGW_AIP4_IRES_EGFP AAV2 6,21E+11 14-Apr-11 Yvonne 25 ul   DD1  
54 pDEST_pTRCGW_Myc_BCAN AAV2 1,43E+12 14-Apr-11 Yvonne 25 ul   DD1  
55 AAV_DIO_Nphr_YFP AAV2 3,79E+12 14-Apr-11 Yvonne 25 ul   DD1  
56 AAV_DIO_YFP AAV2 4,90E+13 14-Apr-11 Yvonne 25 ul   DD1  
57 pAAV-CAMKII-eNpHR3.0-EYFP AAV2 2,78E+12 14-Apr-11 Yvonne 25 ul   DD1  
58 pAAV-CaMKIIa-hChR2(H134R )-EYFP AAV2 6,69E+12 14-Apr-11 Yvonne 25 ul   DD1  
59 pAAV-CamKII-EYFP AAV2 1,26E+13 14-Apr-11 Yvonne 25 ul   DD1  
 
60 pAAV_DIO_ChR2_YFP AAV2 4.26256E+12 28-Jul-11 Yvonne 25 ul   DD1  
61 pDEST_pTRCGW_Nrg3_IRES_EGFP AAV2 1.74167E+13 28-Jul-11 Yvonne 25 ul   DD1  
62 pAAV_CamKii_CREB_GFP_IRES_cre AAV2 6.69649E+12 28-Jul-11 Yvonne 25 ul   DD1  
Line: 86 to 82
 
75 AAV_CamKII_GFP_ires_cre AAV2 2.75E+11 250612 Yvonne 25 ul   DD2  
76 AAV_CamKII_GFP_ires_cre AAV2 5.60E+10 250612 Yvonne 25 ul   DD2  
77 AAV_shisa6_ires_EGFP AAV2 1.11E+10 200712 Yvonne 25 ul   DD2  
Changed:
<
<
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   DD2
>
>
78 AAV_shisa7_ires_EGFP AAV2 3.23E+10 200712 Yvonne 25 ul   DD2  
79   AAV1              

Revision 32012-10-19 - Main.PimVanNierop

Line: 1 to 1
 
META TOPICPARENT name="WebHome"

AAVVirusStocks

Changed:
<
<
<-- /editTable -->
>
>
<-- /editTable -->
 
nr name serotype titer (TU/ml) date made by ul/tube conc.method drawer remarks
Changed:
<
<
C CMV_GFP AAV2 2*10^11   Erich     DD7
C1 CMV_GFP AAV2 3*10^11   Erich     DD7
C2 CMV_GFP AAV8 3*10^11   Erich     DD7
1 CMV_FRZB_IRES_GFP AAV5 3*10^12 12/31/2008 Maarten     DD7
2 H1_sh2 (AIP1AB .2) AAV5 10^13   Yvonne     DD7
3 CMV_mGluR2 AAV5 3*10^10 1/29/2009 Danielle     DD7
4 CMV_IRES_GFP(Harold) AAV5 5*10^11 1/29/2009 Dan/Maarten     DD7
5 CMV_FRZB_IRES_GFP AAV5 1*10^12 1/29/2009 Maarten     DD7
6 CMV_FRZB_IRES_GFP AAV2 2*10^11 3/13/2008 Maarten     DD7
7 CMV_FRZB_IRES_GFP AAV8 5*10^12 3/13/2008 Maarten     DD7
8 CMV_IRES_GFP(Harold) AAV2 2*10^9 3/13/2008 Maarten     DD7
9 CMV_IRES_GFP(Harold) AAV8 3*10^9 3/13/2008 Maarten     DD7
10 CMV_IRES_GFP AAV8 1,1*10^12 5/4/2009 Maarten     DD7
11 WEG       Maarten     DD7
12 CMV_IRES_EGFP (Harold) AAV8   5/4/2009 Maarten 30 ul   DD7
13 pTRCGW_Dest_Brevican_IRES_GFP AAV5 6,6*10^10 5/4/2009 Yvonne     DD7
14 WEG             DD7
15 WEG             DD7
16 pTRCGW_Dest_FLAG_AIP4A_IRES_GFP AAV5 5,8*10^10 5/25/2009 Yvonne 30 ul   DD7
17 CMV_IRES_EGFP(Maarten) AAV8 5,6*10^12 5/25/2009 Maarten 50 ul   DD7
18 CMV_IRES_EGFP (Harold) AAV5   7/8/2009 Danielle 50 ul   DD7
19 CMV_FRZB_IRES_GFP     7/23/2009 Maarten 25 ul   DD7
20 CMV_IRES_EGFP (Maarten) AAV2 6,1*10^11 7/23/2009 Yvonne 25 ul   DD7
21 CMV_IRES_EGFP (Maarten) AAV5 5,5*10^12 7/23/2009 Yvonne 25 ul   DD7
22 pTRCGW_shAIP2_CMV_GFP (sh6) AAV8 8,8*10^10 9/22/2009 Yvonne 10/25 ul   DD7
23 pTRCGW_shAIP4_CMV_GFP (sh12) AAV8 2,2*10^11 9/22/2009 Yvonne 10/25 ul